Thermo Fisher Scientific B7-2 (CD86) Monoclonal Antibody (OTI8C9)

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD86 is one of two ligands (the other being CD80) for CTLA4 and CD28. It acts as a costimulatory molecule in eliciting T-cell help during antigen presentation.
Inadequate co-stimulation involving CD86/CD80 may induce tolerance. CD86 is a type I membrane protein and a member of the immunoglobulin superfamily, expressed by antigen-presenting cells. It interacts with CD28 and CTLA4 on T cells:

• Binding with CD28 provides a costimulatory signal for T-cell activation.
• Binding with CTLA4 negatively regulates T-cell activation, reducing immune response.

Alternative splicing results in two transcript variants encoding different isoforms of CD86. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.