Thermo Fisher Scientific Vimentin Monoclonal Antibody (SP20)
Vimentin 단백질을 인식하는 Rabbit Monoclonal Antibody(SP20)로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. Human, Mouse, Primate 시료에 반응. Recombinant human vimentin을 면역원으로 제작. 연구용으로만 사용.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:500 | 5 publications |
| Immunohistochemistry (IHC) | - | 23 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20 | - |
| Immunocytochemistry (ICC/IF) | 1:1,000 | 8 publications |
| Flow Cytometry (Flow) | 1:100 | - |
| ELISA | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP20 |
| Immunogen | Recombinant protein encoding human vimentin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10981427 |
Product Specific Information
MA5-14564 targets Vimentin in IHC (P), WB, IF, and ICC applications and shows reactivity with Human, Primate, Mouse, and Rat samples.
The MA5-14564 immunogen is a recombinant protein encoding human vimentin.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFPs, vimentin is expressed with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to breakdown of intermediate filaments and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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