Thermo Fisher Scientific Vimentin Monoclonal Antibody (SP20)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Immunocytochemistry (ICC/IF)

1:1,000

View 8 publications 8 publications

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP20

Immunogen

Recombinant protein encoding human vimentin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10981427

Product Specific Information

MA5-14564 targets Vimentin in IHC (P) , WB, IF and ICC applications and shows reactivity with Human, Primate, Mouse and Rat samples.

The MA5-14564 immunogen is recombinant protein encoding human vimentin.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.