Thermo Fisher Scientific IL-27 Monoclonal Antibody (355028)

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

355028

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer Tyr19-Pro228 (EBI3) and Phe29-Ser234 (p28) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-27,uniProtId:O35228-1,ncbiNodeId:10090,antigenRange:19-228,antigenLength:228,antigenImageFileName:MA5-24101_IL-27_O35228-1_Rat.svg,antigenImageFileNamePDP:MA5-24101_IL-27_O35228-1_Rat_PDP.jpeg,sortOrder:3},{targetFamily:IL-27,uniProtId:Q8K3I6-1,ncbiNodeId:10090,antigenRange:29-234,antigenLength:234,antigenImageFileName:MA5-24101_IL-27_Q8K3I6-1_Rat.svg,antigenImageFileNamePDP:MA5-24101_IL-27_Q8K3I6-1_Rat_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610328

Product Specific Information

In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 p28, rmIL-27 EBI-3, or recombinant human (rh) IL-27 heterodimer is observed. In Western blots, no cross-reactivity with rmIL-27 p28 or rhIL-27 heterodimer is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.