Thermo Fisher Scientific SUMO-1 Monoclonal Antibody (SM1, 495)
SUMO-1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry에 적합. Recombinant SUMO1을 면역원으로 제작되었으며, 다양한 종에 반응. Protein A/G 정제, 액상 형태로 4°C 보관.
- 카탈로그번호
- 7341-MSM1-P0
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SUMO-1 Monoclonal Antibody (SM1, 495)
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SM1, 495 |
| Immunogen | Recombinant human SUMO1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
This antibody is predicted to have broad species reactivity.
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or lysine-linked polymer. Covalent attachment via an isopeptide bond requires activation by the E1 complex (SAE1-SAE2), linkage to the E2 enzyme (UBE2I), and promotion by E3 ligases such as PIAS1–4, RANBP2, or CBX4.
This post-translational modification on lysine residues plays a crucial role in nuclear transport, DNA replication and repair, mitosis, and signal transduction.
SUMO1 is involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains may undergo polyubiquitination, signaling proteasomal degradation of modified proteins.
SUMO1 may also regulate genes involved in palate development, and mutations can lead to non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.