Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE, eBioscience
Mouse CD90.2 (Thy-1.2) 단백질을 인식하는 53-2.1 클론의 Rat IgG2a 단일클론 항체로, PE 형광 표지되어 Flow Cytometry에 최적화됨. Thymocytes 및 T세포 표지용으로 사용되며, 0.03 µg/test로 높은 특이성과 재현성을 제공.
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Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.03 µg/test | View 28 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Specification |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465775 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 555
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 760
- NovaFluor Red 710
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains such as BALB/c, CBA, C3H, C57BL/6, C58/, and SJL. Cells from CD90.1-expressing strains (e.g., PL, AKR) do not stain with 53-2.1. CD90 regulates adhesion and signal transduction in T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometry on mouse splenocytes, recommended ≤0.03 µg/test (100 µL final volume).
Cell number per test: 10⁵–10⁸ cells (empirically determined).
Careful titration recommended for optimal assay performance.
Excitation/Emission: 488–561 nm / 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein member of the immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. Thy-1 participates in cell-cell or cell-ligand interactions during synaptogenesis and other brain events. CD90.2 (~18 kDa) is found on hematopoietic cells, neurons, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cells. In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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