Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE, eBioscience
Mouse CD90.2 (Thy-1.2) 단백질을 인식하는 30-H12 클론의 Rat 모노클로날 항체로, PE 형광 표지되어 Flow Cytometry에 최적화됨. T 세포 및 신경세포 표면 분석에 사용되며, 높은 특이성과 안정성을 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE, eBioscience™
Applications
- Reported Use: Flow Cytometric Analysis
- Tested Use: Flow Cytometry (≤ 0.125 µg/test)
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-H12 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465779 |
Additional Formats Available
- Unconjugated
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 780
- Request custom conjugation
Product Specific Information
The 30-H12 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (BALB/c, CBA, C3H, C57BL/6, C58/J, SJL, etc.).
Cells from CD90.1-expressing strains (PL, AKR) do not stain with 30-H12.
CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤ 0.125 µg/test)
A test is defined as the amount (µg) of antibody staining a cell sample in 100 µL final volume.
Cell number range: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal assay performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein of the immunoglobulin superfamily.
It is expressed in murine T cells, thymocytes, neural cells, Kupffer cells, and fibroblasts.
Thy-1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other neural events.
CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic and neuronal cells, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines.
In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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