Thermo Fisher Scientific Cytokeratin Pan Type I/II Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 75 publications 75 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:40

View 15 publications 15 publications

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:40

-

Immunocytochemistry (ICC/IF)

1:100

View 11 publications 11 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Published species

Chicken, Horse, Human, Mouse, Non-human primate, Rat, Sheep, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

AE1/AE3

Immunogen

Human epidermal keratins.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

4°C or -20°C if preferred

Shipping conditions

Wet ice

RRID

AB_2281092

Product Specific Information

Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control is human skin.

This antibody detects the acidic and basic (Type I and II) cytokeratins: Cytokeratin 1, Cytokeratin 2, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 6, Cytokeratin 7, Cytokeratin 8, Cytokeratin 10, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16 and Cytokeratin 19.

Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.