Thermo Fisher Scientific Cytokeratin Pan Type I/II Antibody Cocktail
Pan-Cytokeratin 검출용 AE1/AE3 클론 마우스 모노클로날 항체 칵테일로, 다양한 인간 상피조직 및 종에서 반응. Western blot, IHC, ICC 등 다중 응용 가능. Heat-mediated 항원 회복 권장. 연구용으로만 사용.
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Thermo Fisher Scientific Cytokeratin Pan Type I/II Antibody Cocktail
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:1,000 | 2 |
| Immunohistochemistry (IHC) | – | 75 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:40 | 15 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:40 | – |
| Immunocytochemistry (ICC/IF) | 1:100 | 11 |
| Flow Cytometry (Flow) | – | 1 |
| in situ PLA (PLA) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 2 |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Chicken, Horse, Human, Mouse, Non-human primate, Rat, Sheep, Zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Cocktail |
| Type | Antibody |
| Clone | AE1/AE3 |
| Immunogen | Human epidermal keratins |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2281092 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended for staining of paraffin sections.
- Suggested positive control: human skin.
- Detects acidic and basic (Type I and II) cytokeratins including Cytokeratin 1, 2, 3, 4, 5, 6, 7, 8, 10, 14, 15, 16, and 19.
- Clone AE1/AE3 provides broad reactivity to the 19 known human epidermal keratins.
Target Information
Cytokeratin pan belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity. Represented in human epithelial tissues by at least 20 polypeptides, cytokeratins range in molecular weight between 40–68 kDa and isoelectric pH between 4.9–7.8. Expression patterns vary by epithelium type and differentiation stage, aiding in classification of epithelial malignancies. Cytokeratin antibodies are valuable in tumor differential diagnosis, cytopathology, and flow cytometry. Keratin composition reflects epithelial cell type, growth environment, and disease state, making them useful markers in cancer research and tumor diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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