Thermo Fisher Scientific AXIN2 Monoclonal Antibody (J.415.10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

J.415.10

Immunogen

Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AXIN2,uniProtId:Q9Y2T1-1,ncbiNodeId:9606,antigenRange:566,antigenLength:843,antigenImageFileName:MA5-15015_AXIN2_Q9Y2T1-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15015_AXIN2_Q9Y2T1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

183 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10984109

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Axin1.

Target Information

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.