Thermo Fisher Scientific EIF2S1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,900

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within human EIF2S1 aa 116-315/315. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EIF2S1,uniProtId:P05198-1,ncbiNodeId:9606,antigenRange:116-315,antigenLength:315,antigenImageFileName:PA5-144655_EIF2S1_P05198-1_Rabbit.svg,antigenImageFileNamePDP:PA5-144655_EIF2S1_P05198-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094036

Product Specific Information

Positive control: HepG2 cell lysate, A431 cell lysate, Raji cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse colon tissue lysate, rat spleen tissue lysate, human kidney tissue, Hela, A375, NCI-H441.

Predicted band size: 36 kDa

Subcellular Location: Stress granule.

Target Information

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.