Thermo Fisher Scientific Factor 10 heavy chain Polyclonal Antibody
Factor X heavy chain을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며 Protein A 정제, 1 mg/mL 농도로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Factor X heavy chain (amino acids 351–448) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Factor X undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. The two chains are held together by one or more disulfide bonds. The light chain contains two EGF-like domains, while the heavy chain contains the catalytic domain homologous to other hemostatic serine proteases. The mature factor is activated by cleavage of the activation peptide by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca²⁺, and phospholipid during blood clotting. Mutations in this gene cause factor X deficiency, a hemorrhagic condition of variable severity.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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