Thermo Fisher Scientific Phospho-RACGAP1 (Ser387) Polyclonal Antibody
RACGAP1 단백질의 Ser387 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | — |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | — |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 | — |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | — |
| ELISA | 1:5,000–1:10,000 | — |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human RACGAP1 around the phosphorylation site of Ser387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
RAC, a small GTPase in the Ras family, regulates the formation of membrane ruffles, lamellipodia, and filopodia. It is involved in cytoskeletal actin organization and cellular transformation. The p21-activated kinases (PAKs) are direct targets of active Rac and Cdc42, inducing assembly of polarized cytoskeletal structures in fibroblasts. RAC1 and RAC2 are essential components of NADPH oxidase, responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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