Thermo Fisher Scientific PCNA Polyclonal Antibody
다양한 종에서 반응하는 Thermo Fisher Scientific의 PCNA 폴리클로날 항체로, DNA 복제 및 수선 연구에 적합. Western blot, ELISA, IP 등 다중 응용 가능. 고순도 친화 크로마토그래피 정제, 안정적 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| ELISA | 1:2,000–1:8,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fish, Human, Mouse, Non-human primate, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody from rabbit serum immunized with synthetic peptide corresponding to an internal region of human PCNA protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.97 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening.
Aliquot contents and freeze at -20°C or below for extended storage.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Directed against human PCNA protein. BLAST analysis suggests cross-reactivity with PCNA proteins from human, monkey, dog, mouse, rat, bovine, chicken, fish, and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways.
PCNA binds to CDK inhibitor p21, structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
It serves as a marker for proliferative cells and helps identify tumor proliferation status.
PCNA is localized in the nucleus, acts as a cofactor of DNA polymerase delta, and forms a homotrimer to increase processing during DNA replication.
In response to DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been found, and pseudogenes are described on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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