Thermo Fisher Scientific Phospho-AKT (Ser473) Polyclonal Antibody
Phospho-AKT (Ser473) 특이적 폴리클로날 항체로 Western Blot 및 IHC에 적합. 인간, 생쥐, 랫트 반응성 보유. AKT1/2/3 인산화 형태 인식. 장기 보관 시 -20°C 권장, 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the phospho S473 of mouse AKT (RAC-alpha serine/threonine-protein kinase, PKB, RAC) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen is homologous in rat, human, and chicken AKT1.
- Specificity: AKT1, AKT2, AKT3.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a serine/threonine kinase involved in diverse biological responses such as metabolism regulation, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets like forkhead transcription factors and caspase-9. The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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