Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Super Bright 702, eBioscience
마우스 CD117(c-Kit) 인식용 2B8 단클론 항체로 Super Bright 702 형광체 결합형. 유세포분석에 최적화되어 있으며, 405 nm 레이저로 여기되어 702 nm에서 방출. 혈액세포 및 비혈액세포 성장 인자 연구용. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Super Bright 702, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 21 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 702 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717159 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation available
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor and stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed in hematopoietic progenitor cells, mast cells, and plays a key role in proliferation and differentiation.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells, recommended ≤ 0.125 µg/test.
Super Bright 702 Characteristics:
- Tandem dye excited by 405 nm violet laser, emission at 702 nm
- Recommended 710/50 bandpass filter
- Sensitive to photo-induced oxidation; protect from light
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright dyes
- Fixation compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333)
Laser Information:
Excitation: 405 nm
Emission: 702 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF, stem cell factor). KIT regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon ligand binding, c-Kit dimerizes, activating downstream signaling pathways including Ras/ERK, PI3K, PLC-γ, and JAK/STAT, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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