Thermo Fisher Scientific PIM2 Monoclonal Antibody (OTI10A12), TrueMAB
PIM2 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC(P), Flow Cytometry에 적합합니다. 고순도의 친화 크로마토그래피 정제 항체이며, 동결건조 형태로 제공됩니다. 인체 유래 PIM2 단백질을 면역원으로 제작되었습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10A12 |
| Immunogen | Full length human recombinant protein of human PIM2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PIM2 is a proto-oncogene encoding a serine/threonine kinase that phosphorylates BAD, partially inhibiting its apoptotic function. PIM proteins (PIM-1, PIM-2, PIM-3) are oncogene-encoded kinases with similar oncogenic roles.
PIM-2 is highly expressed in hematopoietic tissues, spleen, thymus, peripheral blood leukocytes, testis, small intestine, and colon. Its expression is negatively regulated by growth factor depletion, and elevated levels have been observed in certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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