Thermo Fisher Scientific TGF alpha Recombinant Rabbit Monoclonal Antibody (JB58-36)
TGF alpha 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. Human 시료에 반응하며, 고순도의 Protein A 정제 항체로 안정적인 신호 검출을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JB58-36 |
| Immunogen | Recombinant protein within Human TGF alpha (aa 1–160) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848588 |
Product Specific Information
Positive Controls:
Human tonsil tissue, human liver tissue, human colon cancer tissue, human kidney tissue, A431, A549, PC-3M.
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independent transformation in cultured cells. Human, murine, and rat TGF-alpha are cross-species reactive. TGF-alpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing a reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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