Thermo Fisher Scientific POLR2J2 Monoclonal Antibody (OTI3G4), TrueMAB
Human POLR2J2 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼 제공. 연구용으로 재구성 및 접합 실험에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G4 |
| Immunogen | Full length human recombinant protein of human POLR2J2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
POLR2J2 is a member of the RNA polymerase II subunit 11 gene family located on chromosome 7q22.1.
This family includes three genes and a pseudogene on chromosome 7p13.
DNA-directed RNA polymerase II polypeptide J encodes a subunit responsible for synthesizing mRNA in eukaryotes.
POLR2J2 produces alternatively spliced transcripts that may encode isoforms with distinct C-termini.
Most variants include additional non-coding exons, making them candidates for nonsense-mediated decay (NMD).
It remains unclear whether this locus expresses a functional protein in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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