Thermo Fisher Scientific PRDX5 Polyclonal Antibody
Human Peroxiredoxin 5(PRDX5)에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 보존제가 없는 액상 형태이며, Protein A 및 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 0.5–2 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Peroxiredoxin 5/PRDX5 protein (Met53–Leu214) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788197 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human Peroxiredoxin 5 (PRDX5).
Target Information
Peroxiredoxin (Prx) is a family of peroxidases involved in cell proliferation, differentiation, and apoptosis. Mammalian Prx proteins, approximately 20–30 kDa in size, are categorized into typical 2-Cys, atypical 2-Cys, and 1-Cys Prx subgroups.
Prx VI belongs to the 1-Cys Prx subgroup, while the other five 2-Cys Prx isoforms exhibit thioredoxin-dependent peroxidase (TPx) activity using thioredoxin, thioredoxin reductase, and NADPH as a reducing system.
Subcellular localization varies: Prx I, II, and VI are found in the cytosol; Prx III in mitochondria; Prx IV in the ER and secretory pathways; and Prx V in peroxisomes, mitochondria, and cytosol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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