Thermo Fisher Scientific RT1.Bu Monoclonal Antibody (OX-3)
Rat Ia 항원을 인식하는 마우스 단클론 항체로, 다양한 랫드 및 마우스 MHC 하플로타입 분석에 사용 가능. IHC(F), Flow Cytometry, IP 등에 적합하며, Protein G 정제 및 PBS 버퍼에 0.02% sodium azide 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | |
| Flow Cytometry (Flow) | 0.02 µg/1x10⁶ cells | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX-3 |
| Immunogen | Rat thymocyte membrane glycoprotein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1087155 |
Available Conjugated Formats
Product Specific Information
The anti-rat RT1.Bu monoclonal antibody recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (though expressed in lower amounts in AO rats), but not BN, DA, or PVG/c strains. It also cross-reacts with mouse strains (MHC haplotypes b and s). Analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9.
This antibody recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. It is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in recognizing donor-origin cells in bone marrow reconstituted radiation chimeras.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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