Thermo Fisher Scientific Cytochrome C Monoclonal Antibody (6H2), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6H2

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865215

Product Specific Information

Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.

Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis, and immunoprecipitation. (Fluorochrome conjugated 6H2 is recommended for use in intracellular flow cytometry.).

Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.