Thermo Fisher Scientific Cytochrome C Monoclonal Antibody (6H2), eBioscience
Mouse monoclonal antibody (clone 6H2) specific for cytochrome C in human, mouse, and rat samples. Suitable for IHC, ICC/IF, Flow Cytometry, and IP. High purity (>90%) and low aggregation (<10%) ensure reliable intracellular detection of apoptotic markers.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Flow Cytometry (Flow) | Assay-dependent | View 2 publications |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865215 |
Additional formats available:
Product Specific Information
The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome C.
Applications Reported:
Used for intracellular flow cytometric analysis and immunoprecipitation. (Fluorochrome-conjugated 6H2 is recommended for intracellular flow cytometry.)
Applications Tested:
Validated by intracellular flow cytometric analysis. Optimal performance achieved by careful titration for each assay.
Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytochrome C is an electron transport protein located in the mitochondrial intermembrane space, essential for oxidative phosphorylation and ATP production.
Release of cytochrome C into the cytosol occurs upon apoptotic stimuli, where it binds to apoptotic protease activating factor, triggering activation of procaspase-9 and subsequently caspase-3—key steps in apoptosis execution.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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