Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody B6H12 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins CD51/CD61 and CD41/CD61. It mediates bi-directional signaling, regulates macrophage phagocytic activity, and serves as a receptor for thrombospondin.

• Expression: Found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic cells.
• Reported Activity: Neutralizing activity.
• Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometry and immunohistochemistry (FFPE sections).
Applications Tested: Pre-titrated for flow cytometric analysis of normal human peripheral blood cells.
Use 5 µL (0.8 µg) per test in a final volume of 100 µL (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures for engineered fluorescent signatures with optimized spillover and spread properties.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and regulates various signaling pathways.
It acts as a receptor for thrombospondin and modulates G protein activation, calcium signaling, and cell adhesion.
CD47 interacts with CD172a (SIRPα) on macrophages to prevent phagocytosis and influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanosignaling, and neuronal development.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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