Thermo Fisher Scientific UBE2E3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.

1. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by the Ub-activating enzyme (E1).
2. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex.
3. The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the appropriate protein substrate.

A wide range of enzymes facilitate the proteolytic Ub pathway, including UBE2E3 (also designated UBCH9), which catalyzes the covalent attachment of ubiquitin to other proteins. UBE2E3 is involved in the regulation of transepithelial sodium transport in renal cells and may also play a role in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in an IPO11-dependent manner. It is ubiquitously expressed at low levels and highly expressed in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.