Thermo Fisher Scientific GPR132 Polyclonal Antibody
Human GPR132 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P) 및 ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됨. PBS 버퍼에 0.1% sodium azide 포함, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 12 µg/mL | |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 18 amino acid peptide from C-terminus of human GPR132 / G2A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550259 |
Product Specific Information
- Percent identity with other species by BLAST analysis:
Human (100%); Gorilla, Marmoset (89%); Gibbon, Monkey (83%). - For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
GPCR G2A is a G-protein linked receptor for lysophosphatidylcholine (LPC).
It may play a role as a sensor of LPC levels at sites of inflammation to limit tissue-infiltrating cells and progression to autoimmune disease.
Functions at the G2/M checkpoint to delay mitosis and may serve as a mechanism for T and B cells, and other cell types, to slow proliferation and repair damaged DNA.
Highly expressed in macrophages and hematopoietic tissues rich in lymphocytes (spleen, thymus), but weakly expressed in heart and lung.
In atherosclerotic plaques, expression is observed around the lipid core and shoulder region.
Induced by DNA-damaging agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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