Thermo Fisher Scientific NLRP3 Polyclonal Antibody
Thermo Fisher Scientific의 NLRP3 Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC/IF, ELISA 등에 사용 가능. Rabbit IgG 기반의 비결합 액상 항체로, NLRP3 인플라마좀 연구에 적합. 고순도 친화 크로마토그래피 정제 및 안정적 저장 용액 제공.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| ELISA | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1–220 of human NLRP3 (NP_001120934.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.15 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805084 |
Product Specific Information
- Positive Samples: U-937
- Cellular Location: Cytoplasm, Endoplasmic reticulum, Inflammasome, Nucleus, Cytosol
Immunogen Sequence:
MKMASTRCKL ARYLEDLEDV DLKKFKMHLE DYPPQKGCIP LPRGQTEKAD HVDLATLMID FNGEEKAWAM AVWIFAAINR RDLYEKAKRD EPKWGSDNAR VSNPTVICQE DSIEEEWMGL LEYLSRISIC KMKKDYRKKY RKYVRSRFQC IEDRNARLGE SVSLNKRYTR LRLIKEHRSQ QEREQELLAI GKTKTCESPV SPIKMELLFD PDDEHSEPVH
Target Information
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif.
It interacts with apoptosis-associated speck-like protein PYCARD/ASC, forming part of the NALP3 inflammasome complex.
This complex acts as an upstream activator of NF-kappaB signaling and regulates inflammation, immune response, and apoptosis.
Mutations are linked to familial cold autoinflammatory syndrome and neonatal-onset multisystem inflammatory disease.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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