Thermo Fisher Scientific Ly-6E Polyclonal Antibody
Ly-6E 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, ICC/IF, ELISA에 사용 가능. Mouse와 Rat 반응성. 고순도 친화 크로마토그래피 정제. -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 21–101 of human LY6E (NP_002337.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.58 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913647 |
Product Specific Information
- Positive test controls: LO2, 293T, SKOV3, MCF7, U-87MG, Mouse brain, Mouse kidney, Mouse liver
- Target localization: Cell membrane, GPI-anchor, Lipid-anchor
Immunogen sequence:
LMCFSCLNQK SNLYCLKPTI CSDQDNYCVT VSASAGIGNL VTFGHSLSKT CSPACPIPEG VNVGVASMGI SCCQSFLCNF S
Target Information
Sca1, also known as Ly6A/E, belongs to the Ly6 multigene family of type V GPI-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in mouse bone marrow of both Ly6.1 and Ly6.2 allotypes.
In peripheral tissues, Sca1 expression varies between allotypes:
- Ly6.1 strains (A, BALB/c, CBA, C3H/He, DBA/1, NZB): few Sca1⁺ resting lymphocytes
- Ly6.2 strains (AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129): higher numbers of Sca1⁺ lymphocytes
Sca1 expression is dramatically upregulated in all strains upon cellular activation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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