Thermo Fisher Scientific Desmoglein-3 Monoclonal Antibody (DSG3, 1535)
Desmoglein-3 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, WB 및 IHC(Paraffin) 실험에 적합합니다. 고순도의 Protein A/G 정제 항체이며, PBS/BSA buffer 내 4°C 보관. 세포 간 접착 및 pemphigus vulgaris 연구에 활용됩니다.
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Thermo Fisher Scientific Desmoglein-3 Monoclonal Antibody (DSG3, 1535)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DSG3, 1535 |
| Immunogen | Recombinant human DSG3 extracellular protein fragment (amino acids 50–615) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is part of the desmosomal cadherin family and plays a critical role in cell-cell adhesion.
It is predominantly expressed in stratified squamous epithelium such as skin, oral mucosa, and esophagus.
Desmoglein 3 is the autoantigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease.
Binding of anti-Desmoglein 3 autoantibodies causes loss of cell-cell adhesion, leading to blister formation and suprabasilar acantholysis.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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