Thermo Scientific™

Pierce™ Quantitative Peroxide Assay Kit (Aqueous

The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (aqueous-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using자세히 알아보기

The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (aqueous-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This aqueous-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit`s formulation includes sorbitol, which provides sensitivity enhancement. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects. This kit provides for maximum sensitivity with non-lipid samples.

Features of the Quantitative Peroxide Assay Kit:

• Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
• Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself

Applications:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Related Products
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
Pierce™ Peroxide Assay Reagent A
Pierce™ Peroxide Assay Reagent B
Pierce™ Peroxide Assay Reagent C

사양

설명Pierce Quantitative Peroxide Assay Kit (Aqueous)

어세이Quantitative Peroxide Assay

검출 방법Colorimetric

제품라인Pierce™

제품 유형Quantitative Peroxide Assay

특이성Not Target-Specific

충분100 Tube Assays or 500 Microplate Assays

용도(애플리케이션)Solution-based Detection, Absorbance

용도(장비)Spectrophotometer, Microplate Reader

수량100 mL

Unit SizeEach