Thermo Fisher Scientific Pierce Quantitative Peroxide Assay Kit (Aqueous

Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Aqueous)

The Thermo Scientific™ Pierce Quantitative Peroxide Assay Kit (aqueous-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This aqueous-compatible peroxide assay kit detects peroxides based on oxidation of ferrous (Fe²⁺) to ferric (Fe³⁺) ions in the presence of xylenol orange. The formulation includes sorbitol, which enhances sensitivity. Most proteins do not interfere with this assay, although some metal chelators may require a blank control to mitigate effects. This kit provides maximum sensitivity with non-lipid samples.

Features

• Peroxides — Detects and measures hydrogen peroxide (H₂O₂) levels in biological and other liquid samples
• Convenient — Ready-to-use kit removes variability, difficulty, and added expense of reagent preparation

Applications

• Measurement of hydrogen peroxide (H₂O₂) in protein samples
• Quantitation of peroxides in detergents
• Assessment of peroxide effects on cellular activity
• Determination of protein glycation
• Measurement of peroxide accumulation in lipids

Assay Principle

In this assay, hydroperoxides convert Fe²⁺ to Fe³⁺ at acidic pH. In the aqueous-compatible formulation, peroxide reacts with sorbitol to form a peroxyl radical, which initiates Fe²⁺ oxidation to Fe³⁺.
In sulfuric acid solution, Fe³⁺ complexes with xylenol orange (XO) dye to yield a purple product with maximum absorbance at 560 nm.
Peroxide levels can be determined using the known extinction coefficient of the XO-Fe complex or by comparison to a standard curve prepared with hydrogen peroxide.

Related Products

• Pierce™ Quantitative Peroxide Assay Kit (Lipid)
• Pierce™ Peroxide Assay Reagent A
• Pierce™ Peroxide Assay Reagent B
• Pierce™ Peroxide Assay Reagent C

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