Thermo Fisher Scientific Pierce Quantitative Peroxide Assay Kit (Lipid)

Thermo Fisher Scientific Pierce™ Quantitative Peroxide Assay Kit (Lipid)

The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (lipid-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This lipid-compatible peroxide assay kit detects peroxides based on oxidation of ferrous (Fe²⁺) to ferric (Fe³⁺) ion in the presence of xylenol orange. The kit’s formulation may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (control) to mitigate these effects.

Features

• Peroxide Detection: Measures hydrogen peroxide (H₂O₂) levels in biological and other liquid samples
• Convenient: Ready-to-use kit eliminates variability and difficulty of reagent preparation

Applications

• General hydrogen peroxide (H₂O₂) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of peroxide effects on cellular activity
• Determination of protein glycation and lipid peroxide accumulation

Principle of the Assay

In this assay, hydroperoxides convert Fe²⁺ to Fe³⁺ at acidic pH. With this lipid-compatible formulation, the peroxide converts Fe²⁺ to Fe³⁺ directly. In a sulfuric acid solution, Fe³⁺ complexes with xylenol orange dye to yield a purple product with an absorbance maximum at 560 nm. Peroxide levels in test samples can be determined using the extinction coefficient of the XO-Fe complex or by comparison to a standard curve prepared with hydrogen peroxide solution.

Related Products

• Pierce™ Quantitative Peroxide Assay Kit (Aqueous)
• Pierce™ Peroxide Assay Reagent A
• Pierce™ Peroxide Assay Reagent B
• Pierce™ Peroxide Assay Reagent C

Specifications