Thermo Fisher Scientific Goat anti-Human IgM Cross-Adsorbed Secondary Antibody, DyLight 650
DyLight 650 형광 표지를 가진 염소 유래 항-인간 IgM 2차 항체로, WB, IHC, ICC, Flow Cytometry 등에 사용 가능. 마우스 및 랫트에 대한 교차 반응 최소화. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgM |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556685 |
| Target | IgM |
| Cross Adsorption | Against mouse and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to mouse and rat.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in detection, sorting, or purification of their specified target. Secondary antibodies offer increased versatility, enabling the use of multiple detection systems such as HRP, AP, or fluorescence. They also enhance sensitivity through signal amplification since multiple secondary antibodies can bind to a single primary antibody. Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 680
- DyLight 755
- DyLight 800
- Custom conjugation available upon request.
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배송/결제/교환/반품 안내
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