Thermo Fisher Scientific Goat anti-Human IgM Cross-Adsorbed Secondary Antibody, DyLight 488

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

1:50- 1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Human IgM

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

View additional formats

• [DyLight 550](/antibody/secondary/product-name/Human IgM Cross-Adsorbed/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Human IgM Cross-Adsorbed/conjugates/DyLight 594/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Human IgM Cross-Adsorbed/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Human IgM Cross-Adsorbed/conjugates/DyLight 680/host/Goat/)
• [DyLight 755](/antibody/secondary/product-name/Human IgM Cross-Adsorbed/conjugates/DyLight 755/host/Goat/)
• [DyLight 800](/antibody/secondary/product-name/Human IgM Cross-Adsorbed/conjugates/DyLight 800/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556682

Target

IgM

Cross Adsorption

Against mouse and rat

Antibody Form

Whole Antibody

Product Specific Information

This antibody is cross-adsorbed and exhibits minimum reactivity to mouse and rat.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.