Thermo Fisher Scientific F(Ab`)2 Fluorescein Polyclonal Antibody, Biotin
Goat 유래 Polyclonal 항체로 Fluorescein에 결합된 Biotin 표지형. WB, IHC, ELISA 등 다양한 응용 가능. 높은 특이성과 안정성으로 연구용 형광 검출에 적합. 장기 보관 시 -20°C에서 안정 유지.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fluorescein conjugated to Goat IgG |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum and Fluorescein conjugated BSA.
No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to emit visible yellow-green light.
Fluorochrome labeling enables rapid and accurate localization of antigen-antibody interactions.
FITC is widely used as a marker for antibodies in immunofluorescent techniques and as a hapten to label proteins.
Anti-FITC antibodies can be used for isolating FITC-labeled cells by magnetic microbeads-based sorting and for reducing background signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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