Thermo Fisher Scientific F(Ab`)2 Biotin Polyclonal Antibody, AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (IHC)

1:500-1:1,000

-

ELISA (ELISA)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Conjugate

AP AP AP

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

0.05M tris HCl, pH 8, with 0.0001M ZnCl2, 50% glycerol, 0.15M NaCl, 10mg/mL BSA, 0.001M MgCl2

Contains

0.01% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Biotinylated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.