Thermo Fisher Scientific CD47 Monoclonal Antibody (miap301), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 1 µg/test
  • View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• Functional Grade
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody miap301 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein with a small cytoplasmic domain. It is involved in adhesion through interactions with SIRP (signal regulator protein) and is associated with β3 integrins (CD51/CD61, CD41/CD61). CD47 also serves as a receptor for thrombospondin.

Expression is found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and non-hematopoietic cells. No crossreactivity is seen against human CD47.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume). Optimize titration for best results.

Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation: Samples can be stored in:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  for up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions. It modulates signaling pathways such as activation of heterotrimeric G proteins and increases intracellular calcium upon adhesion.

CD47 serves as a receptor for the C-terminal domain of thrombospondin and plays roles in membrane transport and signal transduction. It has broad tissue distribution and acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells. The CD47-CD172a system also influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, especially in synapse-rich brain and retinal regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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