Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor dyes, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine5.5, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright series, and custom conjugation request available.

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It plays a crucial role in lymphocyte activation via its tyrosine phosphatase enzymatic activity.

• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Optimal titration recommended.
• Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
• Excitation/Emission: 488–561 nm / 775 nm; compatible with Blue, Green, and Yellow-Green lasers.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and plays key roles in T- and B-cell receptor signaling, cytokine receptor modulation, and integrin-mediated signal transduction. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 is essential for lymphocyte development and immune regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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