Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience
Mouse CD45 단백질을 인식하는 30-F11 클론의 Rat IgG2b 단일클론 항체로, PE-Cyanine7 형광체 결합. Flow cytometry에 최적화되어 있으며, 0.125 µg/test로 사용 가능. 모든 혈액세포에서 발현되는 CD45 검출에 활용. 빛에 민감하며 4°C 암소 보관.
- 카탈로그번호
- 25-0451-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience
Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry | 0.125 µg/test | View 325 publications |
| Miscellaneous PubMed | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716950 |
Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor dyes, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine5.5, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright series, and custom conjugation request available.
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It plays a crucial role in lymphocyte activation via its tyrosine phosphatase enzymatic activity.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Optimal titration recommended.
- Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
- Excitation/Emission: 488–561 nm / 775 nm; compatible with Blue, Green, and Yellow-Green lasers.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and plays key roles in T- and B-cell receptor signaling, cytokine receptor modulation, and integrin-mediated signal transduction. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 is essential for lymphocyte development and immune regulation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.