Thermo Fisher Scientific HK2 Monoclonal Antibody (3D3)
Human HK2 단백질을 특이적으로 인식하는 Mouse monoclonal antibody (Clone 3D3). Western blot, IHC, ICC/IF, Flow cytometry, ELISA에 사용 가능. 인슐린 반응 조직에서 발현되는 Hexokinase 2 검출에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | – |
| ELISA | 1:10,000 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D3 |
| Immunogen | Purified recombinant fragment of human HK2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10986812 |
Product Specific Information
MA5-15679 targets HK2 in indirect ELISA, FACS, IHC, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human HK2 expressed in E. coli.
MA5-15679 detects HK2, which has a predicted molecular weight of approximately 102 kDa.
Target Information
Hexokinase (HK) enzymes catalyze the first step of glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate, thereby committing glucose to the glycolytic pathway.
There are four major isoenzymes: HK1, HK2, HK3, and HK4.
Hexokinase 2 is the predominant isoform in insulin-responsive tissues such as skeletal muscle.
Its expression is insulin-regulated, and studies in rats suggest involvement in the elevated glycolysis rate observed in rapidly growing cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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