Thermo Fisher Scientific HK2 Monoclonal Antibody (3D3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D3

Immunogen

Purified recombinant fragment of human HK2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10986812

Product Specific Information

MA5-15679 targets HK2 in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples.

The MA5-15679 immunogen is purified recombinant fragment of human HK2 expressed in E. Coli.

MA5-15679 detects HK2 which has a predicted molecular weight of approximately 102kDa.

Target Information

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.