Invitrogen™

GeneArt™ Linear pUC19L Vector for Seamless Cloning

The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7자세히 알아보기

The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt™ Seamless Cloning and Assembly Kit (Cat. No. A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.

Some of the key features and elements of the pUC19L vector include:

• lacZα gene allows easy blue/white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit

사양

제품 유형Cloning Vector

리포터 유전자Beta-Gal (lacZ)

항생제 내성 박테리아Ampicillin (AmpR)

복제 개시점pUC Origin

배송 조건Dry Ice

크기 범위13 kb total (vector plus all inserts)

클로닝 방법Seamless Cloning

농도50 ng/mL

수량20 Reactions

벡터Seamless Cloning Vectors

제품라인GeneArt™

Unit SizeEach