Thermo Fisher Scientific Microphthalmia Transcription Factor (MITF) Monoclonal Antibody (PCRP-MITF-1D9)
MITF 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, ICC/IF, Flow cytometry, IP, Peptide array 등에 사용 가능. 인간에 반응하며, 단백질 A/G 정제, 액상 형태, 4°C 보관. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Immunoprecipitation (IP) | 1–2 µg |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-MITF-1D9 |
| Immunogen | Recombinant full-length human MITF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Predicted to react with Chick, Mouse, Rat, Xenopus, and Zebrafish.
Target Information
Mi (Microphthalmia-associated transcription factor, MITF) is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor involved in pigmentation, mast cell function, and bone development. Mutations in Mi cause Waardenburg Syndrome type II in humans. In mice, loss of Mi results in depigmentation, osteopetrosis, and defects in natural killer and mast cells.
There are two known isoforms of Mi differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes (52–56 kDa), while longer forms (60–70 kDa) are found in osteoclasts, B16 melanoma cells, mast cells, and heart tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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