Thermo Fisher Scientific Aquaporin 2 Monoclonal Antibody (5B6B12)
Aquaporin 2 단백질을 인식하는 Mouse monoclonal antibody로, Western blot 및 ELISA 검증 완료. 1 mg/mL 농도의 액상 형태이며 Protein G로 정제됨. 인간 시료에 반응하며, 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B6B12 |
| Immunogen | Purified recombinant fragment of human AQP2 (AA: 149–271) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787255 |
Product Specific Information
MA5-31631 has been tested in indirect ELISA.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct.
Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans.
A vasopressin-induced cAMP increase results in phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells.
Serine-261 has also been identified as a phosphorylation site, with levels decreasing under vasopressin treatment, suggesting its role in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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