Thermo Fisher Scientific IL-10 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

-

ELISA (ELISA)

1:10,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-10,uniProtId:P22301-1,ncbiNodeId:9606,antigenRange:1,antigenLength:178,antigenImageFileName:PA5-85660_IL-10_P22301-1_Rabbit.svg,antigenImageFileNamePDP:PA5-85660_IL-10_P22301-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792799

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Rhesus Monkey (100%), Chimpanzee (100%).

Positive Control: 293T, human IL10-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.