ELK Biotechnology V-ATPase B1 rabbit pAb
V-ATPase B1 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, IF, ELISA에 사용 가능. 인간 ATP6V1B1 유래 펩타이드로 면역생성. 세포막 위치 단백질 검출에 적합하며, -20°C에서 1년 보관 가능.
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제품명
V-ATPase B1 rabbit pAb
제품 정보
| 항목 | 내용 |
|---|---|
| Alternative Names | ATP6V1B1; ATP6B1; VATB; VPP3; V-type proton ATPase subunit B; kidney isoform; V-ATPase subunit B 1; Endomembrane proton pump 58 kDa subunit; Vacuolar proton pump subunit B 1 |
| Applications | WB; IHC; IF; ELISA |
| Recommended Dilutions | Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 ELISA: 1/5000 Not yet tested in other applications |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATP6V1B1 (AA range: 381-430) |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 60 kDa |
| GeneID (Human) | 525 |
| Human Swiss-Prot No. | P15313 |
| Cellular Localization | Apical cell membrane; Basolateral cell membrane |
| Species Reactivity | Human; Mouse |
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase-dependent organelle acidification is essential for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain includes three A and three B subunits, two G subunits, and the C, D, E, F, and H subunits, containing the ATP catalytic site. The V0 domain consists of five subunits: a, c, c’, c’’, and d. Multiple isoforms of V1 and V0 subunits are encoded by distinct genes or alternatively spliced variants. This encoded protein is one of two V1 domain B subunit isoforms.
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