Thermo Fisher Scientific Phospho-Doublecortin (Ser335) Polyclonal Antibody
Doublecortin 단백질의 Ser335 인산화 형태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 IHC(Paraffin)에 적합하며, 1 mg/mL 액상 형태로 제공. 신경세포 이동 연구 및 미세소관 조절 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DCX (Accession O43602), corresponding to amino acid residues around phosphorylated Ser335. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2815939 |
Product Specific Information
This antibody detects endogenous levels of Doublecortin only when phosphorylated at Ser335.
Target Information
In the developing cortex, cortical neurons must migrate over long distances to reach their final differentiation site.
Doublecortin (DCX) is a cytoplasmic protein that regulates neuronal migration by organizing and stabilizing microtubules.
It contains two doublecortin domains that bind microtubules and interacts with LIS1, the regulatory gamma subunit of platelet-activating factor acetylhydrolase.
This interaction is crucial for proper microtubule function in the developing cortex.
Mutations in the DCX gene cause X-linked lissencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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