
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 Goat anti-Mouse IgG (H+L) Secondary Antibody, AP는 마우스 IgG 검출용으로 제작된 고감도 폴리클로날 항체입니다. Western blot, IHC, ELISA, IP 등에 적합하며, AP 결합으로 효소 기반 검출에 활용됩니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공합니다.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:50,000 |
| Immunohistochemistry (IHC) | 1:5,000–1:50,000 |
| ELISA | 1:5,000–1:50,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Mouse IgG, whole molecule |
| Conjugate | AP |
| Form | Lyophilized |
| Concentration | 0.6 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 8.0, with 15 mg/mL BSA, 50 mM sucrose |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_10959407 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #31321 has been successfully used in Western blot, IHC, and IP applications.
This antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins.
It may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend shelf-life, add an equal volume of glycerol (final concentration ~50%) and store at -20°C.
Reconstitute with 0.2 mL of distilled water (1 mg/mL after restoration).
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of mouse targets.
Secondary antibodies offer versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification.
Typically, these antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or other modification methods to ensure high specificity.
Additional Formats Available
- Agarose
- Alexa Fluor 488, 555, 647, 680, 790
- Alexa Fluor Plus 405, 488, 647
- bGal
- Biotin
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 800 4X PEG
- FITC
- HRP
- PE
- Rhodamine
- TRITC
- WesternDot 625
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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