Thermo Fisher Scientific Phospho-NFkB p65 (Ser536) Recombinant Rabbit Monoclonal Antibody (NFkBS536-C8)
NFkB p65 (Ser536) 인산화형을 특이적으로 인식하는 재조합 토끼 단클론 항체로, Western blot에 최적화됨. 높은 특이성과 민감도, 일관된 로트 간 품질을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 연구용에 적합.
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Applications
- Western Blot (WB): 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | NFkBS536-C8 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser536 of human phospho NFkB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897034 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and located on chromosome 11 in humans. Also known as RelA, it belongs to the Rel family of proteins and forms a heterodimer with p50 or p52 subunits.
NF-kB plays a key role in transcriptional regulation of immune-related genes. Binding of TNFα to its receptor activates IKK, leading to phosphorylation and degradation of IκB, thereby releasing NF-kB to translocate to the nucleus and induce gene expression.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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