Thermo Fisher Scientific Human PCSK9 (NARC1) His-tag Recombinant Protein
Human PCSK9 (NARC1) His-tag 재조합 단백질로, HEK293 세포에서 발현된 고순도(>95%) 단백질입니다. ELISA, WB, IHC, 기능 분석 등에 사용 가능하며, LDLR 결합 활성 검증 완료. 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human PCSK9, Met-1–Gln692 (Accession # NP_777596.1) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 72.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Recombinant Human LDLR/LDL Receptor Protein (mFc Tag on ProA Biosensor) binds Human PCSK9 (NARC1) His-tag Recombinant Protein with an affinity constant of 16 nM (BLI assay, Sartorius Octet RED384, QC tested) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
- Reconstitution: Dissolve in sterile double distilled water to ≤0.2 mg/mL.
- Aliquoting: Recommended to aliquot and use promptly.
- Storage: Store aliquots under sterile conditions at -20°C.
- Handling: Avoid repeated freeze-thaw cycles.
Target Information
PCSK9 is a proprotein convertase of the proteinase K subfamily within the secretory subtilase family. It is synthesized as a soluble zymogen and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. PCSK9 plays a key role in cholesterol homeostasis and may contribute to cortical neuron differentiation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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