Thermo Fisher Scientific Human Fc gamma RIIIA (CD16a) (176 Phe) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD16a, amino acids Met1-Gln208 (Accession # P08637-1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

23.3 kDa

Class

Recombinant

Type

Protein

Purity

>95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC

Endotoxin concentration

<1.0 EU/µg

Activity

1.Loaded Human Fc gamma RIIIA (CD16a) (176 Phe) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.9 µM as determined in a BLI assay (Sartorius Octet Red384) (QC tested).
2.Captured Human Fc gamma RIIIA (CD16a) (176 Phe) His-tag Recombinant Protein on Anti-His Chip can bind Human IgG Fc with an affinity constant of 7.1 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
3.Captured Bevacizumab (IgG1) on proA Chip can bind Human Fc gamma RIIIA (CD16a) (176 Phe) His-tag Recombinant Protein with an affinity constant of 2.67 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
4.The purity of Human Fc gamma RIIIA (CD16a) (176 Phe) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 36-48 kDa verified by SEC-MALS.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.