Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI2C12), TrueMAB
ATG3 단백질을 표적하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합. HEK293 유래 full-length human ATG3 항원으로 제작. 높은 특이성과 안정성, 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C12 |
| Immunogen | Full-length human recombinant ATG3 protein produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.39 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ATG3 (APG3) is a widely expressed conjugating enzyme essential for APG8 lipidation, a critical step in autophagy initiation. Autophagy is a process of bulk degradation of cellular proteins through the autophagosomal-lysosomal pathway, vital for normal growth control and nutrient preservation under starvation. It is negatively regulated by TOR through phosphorylation of APG1. ATG3 acts as an E2-like enzyme during autophagosome formation by catalyzing the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate formation, which is crucial for autophagy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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