Thermo Fisher Scientific Streptavidin Protein, APC

Applications

Western Blot (WB)

• Tested Dilution: Assay-dependent

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 73 at 650 nm for 1% solution for APC molecules bound to at least one active streptavidin)
• Allophycocyanin Source: Spirulina platensis
• Working Dilution Range: 1:50–1:200 (most applications)
• Absorption Maximum: 650 nm
• Emission Maximum: 660 nm
• Restoration and Storage:
  • Store freeze-dried product at 4°C until reconstitution.
  • Restore with 0.5 mL distilled water.
  • Centrifuge if not clear after reconstitution; check clarity using a Pasteur pipette.
  • Store at 4°C. Do not freeze.
  • After dilution, use within one day.
• Country of Origin: USA

Target Information

Streptavidin은 Streptomyces avidinii에서 정제된 4량체 단백질로, 비타민 비오틴(biotin)과 매우 강하게 결합(Kd ~10⁻¹⁴ mol/L)합니다. 이러한 높은 결합 친화도는 스트렙타비딘을 진단 및 실험 키트의 핵심 구성요소로 만듭니다.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.