Thermo Fisher Scientific Streptavidin Protein, APC

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Type

Protein

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 73 at 650 nm for a 1% solution for only those APC molecules to which at least one molecule of active streptavidin is bound)

Allophycocyanin Source: Spirulina platensis

Working Dilution Range: 1:50-1:200 for most applications. Absorption Maxima: 650 nm

Emission Maximum: 660 nm

Restoration and Storage: Store freeze-dried product at 4°C until reconstitution. Restore with 0.5 mL distilled water. Centrifuge product if it is not completely clear after reconstitution. To judge clarity, draw product into a pasteur pipette. Store at 4°C. [Do not freeze]. After dilution, do not use for more than one day.

Country of origin: USA

Target Information

Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.