Thermo Fisher Scientific Streptavidin Protein, PE

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

1:50-1:200

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Type

Protein

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm

Form

Lyophilized

Concentration

0.5 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 82.0 at 566 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active streptavidin is bound)

Phycoerythrin Source: Seaweed

Restoration and Storage: The expiration date is six months from date of reconstitution. Store freeze-dried product at 4°C until reconstitution. Restore with 1.0 ml distilled water. Centrifuge product if it is not completely clear after reconstitution. To judge clarity, draw product into a pasteur pipette. Store at 4°C. [Do not freeze]. After further dilution of the reconstituted streptavidin, do not use for more than one day.

Country of origin: USA

Target Information

Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.