Thermo Fisher Scientific CD15/FUT4 Monoclonal Antibody (FUT4, 815)

Applications

• Immunohistochemistry (Paraffin) (IHC (P))
  Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types, including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis x and sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation via calcium-dependent Le(x)-Le(x) interactions, involved in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is widely used in confirming Hodgkin’s disease diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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