Thermo Fisher Scientific CD15/FUT4 Monoclonal Antibody (FUT4, 815)
CD15/FUT4 단일클론 항체로 인간, 마우스, 랫트 시료에 반응. 포르말린 고정 조직의 IHC(P) 분석에 적합. 단백질 A/G 정제, 보존제 무첨가 PBS 용액 형태. 호지킨병 진단 연구 등 세포 표지에 활용 가능.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FUT4, 815 |
| Immunogen | Purified human neutrophils |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry on PFA-fixed tissues, incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD15 (Lewis X, Le(x); stage-specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on various glycolipids, glycoproteins, and proteoglycans of many cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) expression exhibit strong calcium-dependent self-aggregation, involved in processes like embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and its isomer sialyl-Le(a) serve as selectin ligands. CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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