Thermo Fisher Scientific CD15/FUT4 Monoclonal Antibody (FUT4, 815)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• For immunohistochemistry on PFA-fixed tissues, incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD15 (Lewis X, Le(x); stage-specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on various glycolipids, glycoproteins, and proteoglycans of many cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) expression exhibit strong calcium-dependent self-aggregation, involved in processes like embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and its isomer sialyl-Le(a) serve as selectin ligands. CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.